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Aerosol Technology Laboratory (ATL) at BAEN
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Aerosol Sampling

Wetted-Wall Cyclone (WWC) Sampling Systems

  • Three autonomous scaled systems:

    • 100 L/min

    • 400 L/min

    • 1250 L/min

  • Concentration Factor: ∼ 10 ^ 6
  • Cut Point: ∼ 1 µm AD
  • Low Cut Point (LCP): < 500 nm AD

 

Low Cutpoint LCP-WWC- How It Works

  1. Collection of aerosol particles starts with particle laden air being drawn into the Air Inlet.
  2. Atomized collection fluid is injected into the air stream.
  3. The Collection fluid and particles impact on a tangential collection surface, entraining the particles in liquid.
  4. Air shear continuously transports the fluid from the collection surface toward the exhaust. The liquid sample is skimmed off and continuously extracted at thesample outlet.
  5. The air flow then exits the exhaust.

High collection efficiency for Polystyrene Latex beads (PSL) and bioaerosols (virus, bacteria, fungi)

 

 

Sampling in Mass Transit Center

  • WWC and dry filter sampler collocated
  • Sampled over 3 days
  • Collected samples 3x/day

4 logs more bacterial colonies from WWC versus dry filter collector.

Sampling in Poultry Facility

  • WWC and dry filter sampler collocated
  • Sampled over 5 days
  • Collected samples 3x/day

 

  • PCR for pathogens
  • Staph A, B, and C
  • Salmonella 
  • Campylobacter

Dramatic increase in pathogen level detected after hosing floor.

 

Sampling at Corn Harvesting and Processing

WWC to sample for the fungus Aspergillus flavus (A. flavus), in cornfields and in a corn “Shelling Room” at Texas A&M. A. flavus produces the harmful aflatoxin.

The WWC identifies spikes in spore concentration during operations

 

Sampling for Swine Influenza Virus (SIV)

  • Simple straightforward WWC air samples
  • “Just work” at the push of a single button
  • Months of demanding testing in rural Ohio

 

Real-Time Microfluidic Nano-Aerosol Monitoring System in Large Air Spaces

  • Microchannel coupled with the WWC
  • For nanoparticles 150 µg – 15 mg/mL

 

Residential Shower Studies

  • WWC in shower
  • 5600 fungal spores/liter air
  • Intensive cleaning reduces level by only 30%
  • Lung pathogens detected

 

 

 

 

 

 

Experimental Shower Studies

Samples: bioaerosol, biofilm, water

 

 

 

 

 

 

Development of diverse biofilms

 

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